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Compensating anisotropy in histological serial sections with optical flow-based interpolation

Histological serial sections allow for 3D representation of anatomical structures in microscopic to mesoscopic range. However, due to the nature of the acquisition, they suffer from severe anisotropy: 14-to-1 in a single average microscopic paraffin section. We present an interpolation method based on optical flow and show that standard interpolation methods are less suited for serial sections. With our non-linear interpolation approach we are able to represent the "movement" of image parts that are of interest. This allows for better 3D reconstructions and further insights in microanatomy.

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